Flow Cytometry

Fiber Coupled Lasers

Flow Cytometry is a tool for measuring cells and particles, often the cells or particles are suspended in a fluid and have a fluorescence marker attached. As the cells (cell+marker ) stream through various laser wavelengths, they may emit a fluorescent energy signal which can be analyzed by high speed, low noise detection systems.  The target zone for detection is often a rectangular area in the 10’s of microns.  Because of the speed and size of the particles, and small detection area, flow cytometer instrumentation is particularly sensitive to laser power stability, noise, beam wander, and thermal instabilities.

Fiber coupled laser diode modules (FiberTec™ laser module) are well suited to this application because of their inherent power stability, low noise, ability to remotely locate the laser from the optical system (improving system stability and reducing instrument size), superior beam pointing stability and long laser lifetime.

Often a 1st order optical system is used to combine free space beams of several lasers into one collinear path, and project the multiple beams onto the target area.  Red (635nm up to 670nm), blue(typically 488nm), green (532nm), yellow (561nm), and violet (405nm) are the most common wavelengths. Another multi-beam technique is to combine the lasers into a single mode fiber, and then collinear beams coming out of the fiber onto the illumination target area.  SpectraTec™ X has the ability to combine up to 4 unique laser wavelengths into a single mode fiber, which can then be collimated and projected on the target area.

If particle size is of importance the instrumentation will often use polarized light sources for scattering measurements and calculate particle size or use electric fields to “sort” the different sized particles. In this case, a polarized free space laser system, such as the MiniLase™ or ChromaLase™ is recommended.

Laser Applications

The Blue Sky Research offers several OEM laser modules and systems which are easily integrated into a variety of OEM platforms; these include fiber coupled laser modules, Multi-laser modules (Laser Engines)  and free space laser modules with customizable launch & relay optics. Integrated firmware and computer control make application development easy and practical, small footprint, direct diode modulation and low power consumption make our laser modules ideal for instrumentation applications where power, noise and heat generation are major design challenges.

Explore the many ways in which Blue Sky Research is powering dozens of commercial opportunities, read about some Industry Applications below:

Raman Spectroscopy

Raman Spectroscopy used in Pharmaceutical R&DRaman spectroscopy is a non-destructive technique that employs laser light to examine intermolecular bonds and molecular vibrations. This method yields insights into the composition and structure of gases, liquids, or solids. Portable devices utilize compact, low-power Raman lasers, either fiber-coupled (FiberTec SF 638nm, 785nm or 830nm) or free-space (NECSEL Novatru Chroma 633, 785 or 785+830), for rapid identification of substances like drugs or pollutants in the field. In benchtop setups, low to medium-power lasers, also fiber-coupled (FiberTec SF 638nm, 785nm or 830nm) or free-space (NECSEL Novatru Chroma 633, 785 or 785+830), serve various applications from pharmaceuticals to materials science, even extending to biology, manufacturing controls, and nanotechnology. Advanced research setups employ higher-power free-space lasers (NECSEL NovaTru Chroma 785/830 High Pout) to delve into intricate molecular dynamics across a wide range of applications.

Confocal Microscopy

Drug discovery and development activities are accelerated using  confocal laser scanning microscopy to understand the biology of living cells.  The image resolution in the sample depth direction, is much better than that of wide-field microscopes. However this increased resolution is at the cost of decreased signal intensity so long exposures or higher powers are often required.  To obtain 2D or 3D images, the system often use a raster scan technique.  Fiber Coupled lasers offer excellent beam quality and stability as well good power levels for this application; in addition the offer the designer the added option of remotely locating lasers sources.  The ChromaLase™ family offer powerful high quality gaussian beams which can then be beam combined and passed through an AOM (acoustic optic modulator).  Read More >>

Life Sciences

As the population ages, and the demands for clean food and water increase the Life Science, Medical and Biotechnology fields have seen a rapid increase in demand for instrumentation and technology which supports human health, drug discovery, cancer research and blood screening and DNA sequencing.  Small ultra stable laser diodes, like the MiniLase™ Plus, are particularly useful in these applications, the required wavelengths range from UV (370nm) in flow cytometers to NIR for blood/oxygen analysis or retinal mapping for Lasik surgery.  Read More about other Life Science laser applications >>

Food Safety

Lasers are often used in food safety instrumentation for the purposes of typically detecting salmonella, Coliform, E.coli, Enterobacteriaceae, and Yeast, in both raw materials and finished products.  Similar to flow cytometer instruments, fluorescence (direct or by marker) is the typical detection scheme, however 1-2 wavelengths are often sufficient for these systems.  Because of the industrial setting (EX: vineyard, dairy farm, or refrigerated warehouse) power stability and low noise are often key metrics while volume commercial  applications require products to meet aggressive price-to-value targets.  OEM MiniLase™ laser modules or the Industrial grade MiniLase™​ Plus will work for the 635nm requirements and offer excellent performance-value.  The ChromaLase™488nm product is the best solution for 488nm radiation, because it offers superior performance and value relative to non-semiconductor laser technologies (Ar ion lasers, direct frequency doubling, or  optically pumped semiconductors).  Read More >>

Microelectronics

Laser applications in the Microelectronics industry are broad and diversified. Various laser technologies are integrated into major semiconductor processes, including laser cutting, drilling, welding/bonding, debonding, marking, patterning, marking, measurement, and deposition. Of these, laser marking is one of the most common applications for low power lasers. Typically, low power NIR and Green lasers are used in Etching or Annealing semiconductors. We would recommend that you define laser power levels, wavelengths, and and thermal stability as ket factors when choosing whether a free space laser solution (MiniLase™​ Plus or ChromaLase™), or a remotely located Fiber Coupled laser solution is most appropriate for your performance requirements.